five lane mouse rotarod  (Med Associates Inc)

Journal: iScience

Article Title: The GPCR Smoothened on cholinergic interneurons modulates dopamine-associated acetylcholine dynamics, learning, and effort management

doi: 10.1016/j.isci.2026.115324

Figure Lengend Snippet: Smo bidirectionally impacts motor learning (A) Structure of individual rotarod trials, performed 10 times per day. (B) Average latency to fall ( top ) and best-fit performance curves to criterion ( middle and bottom ) for Smo L/L :ChATCre +/− ( left, bottom ) and SmoM2 C/- :ChATCre +/− ( right, bottom ) versus respective controls (middle). (C) Average slope of individual best-fit performance curves for Smo L/L :ChATCre +/− and control mice ( n = 8 per genotype; Shapiro-Wilk test indicated lack of normality, p < 0.01; Mann-Whitney U test, p > 0.05). (D) Average number of days to reach criterion for Smo L/L :ChATCre +/− and control mice ( n = 8 per genotype; Brown-Forsythe test indicated unequal variances, p < 0.01; Welch-corrected unpaired two-tailed t test, ∗ p < 0.05). (E) Average slope of individual best-fit performance curves for SmoM2 C/- :ChATCre +/− and control mice ( n = 9–10 per genotype; F test indicated unequal variances, p < 0.01; Welch-corrected unpaired two-tailed t test, p > 0.05). (F) Average number of days to reach criterion for SmoM2 C/- :ChATCre +/− and control mice ( n = 9–10 per genotype; Shapiro-Wilk test indicated lack of normality, p < 0.01; Mann-Whitney U test, p > 0.05). (G) Slope comparison between control mouse strains ( n = 8–9 per strain; Shapiro-Wilk test indicated lack of normality, p < 0.01; Mann-Whitney U test, p > 0.05). (H) Best-fit slopes normalized to background strain ( n = 8–17 per genotype; Shapiro-Wilk test indicated lack of normality, p < 0.01; Kruskal-Wallis test, ∗ p < 0.05; post hoc Dunn’s multiple comparisons test: ∗ p < 0.05). (I) Average latency to fall post-criterion, normalized to background strain ( n = 8–17 per genotype; one-way ANOVA: main effect, F(2, 26) = 1.36, p > 0.05).

Article Snippet: Trials were conducted in a room with low ambient light and noise using a five-lane mouse rotarod (ENV-574M; Med Associates) programmed to accelerate linearly from 4 to 40 RPM over 300 seconds (preset speed setting #9).

Techniques: Control, MANN-WHITNEY, Two Tailed Test, Comparison

Journal: Frontiers in Cellular Neuroscience

Article Title: Complement C4-deficient mice have a high mortality rate during PTZ-induced epileptic seizures, which correlates with cognitive problems and the deficiency in the expression of Egr1 and other immediate early genes

doi: 10.3389/fncel.2023.1170031

Figure Lengend Snippet: Comparison of motor and cognitive functions of unmanipulated WT, C3-, and C4B-deficient mice. Unmanipulated WT, or C3 –/– , or C4B –/– mice were assessed for their performance in Rotarod (A) and Barns maze (B,C) tests as described in Section “2. Materials and methods”. In panel (A) , the time to fall from the rotating rod is shown for WT, or C3 –/– , or C4B –/– mice in the Rotarod test on day 1, day 2, day 3, day 4, and day 5. The mean ± standard error (SE) of time to fall (y-axis) vs. day of training (x-axis) is shown [C3 –/– vs. WT: ** p < 0.01, C4 –/– vs. WT: *** p < 0.001; two-way ANOVA, C4 –/– vs. WT: F (4,16) = 13.90; C3 –/– vs. WT: F (4,16) = 6.439; C4 –/– vs. C3 –/– : not significant; n = 3 mice]. In panels (B,C) , the latency time is shown for WT, or C3 –/– , or C4B –/– mice in the Barns maze test during the training period on day 1, day 2, day 3, and day 4 (B) , and the final trial on day 5 (C) . In panel (B) , the mean ± standard error (SE) of latency time (y-axis) vs. day of training (x-axis) is shown. The indicated difference on day 4 was statistically significant, as determined by one-way ANOVA followed by Tukey post-hoc tests [* p < 0.05 for comparisons C4 –/– vs. WT and C4 –/– vs. C3 –/– on day 4; n = (4 mice × 3 trails) = 12]. In panel (C) , the median with 10%/90% percentiles of individual mice are shown in a box and whisker plot with the mean value indicated by “+”. The indicated differences were statistically significant for the final trial on day 5, as determined by one-way ANOVA followed by Tukey post-hoc tests [** p < 0.01 for comparisons between two groups; F (2,9) = 23.56; n = 4 mice].

Article Snippet: To test motor functions, we utilized a standard five-lane mouse Rotarod set-up (Med Associates Inc., Fairfax, VT, USA) with programmed accelerated rotation (4–40 rpm).

Techniques: Comparison, Mouse Assay, Whisker Assay